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Cell proliferation assays for research proposal

Cell proliferation assays for research proposal


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I'm an undergrad Biology student and I'm working on a research proposal for a certain course. I need help or guide on cell proliferation assays. I have a target gene , recent papers show that this gene's haploinsufficiency increases cell proliferation. However the most recent paper (published in 2020) showed that haploinsufficency decreases cell proliferation instead. I want to investigate this discrepancy but I don't know what methods should I use. In both papers they used immunostaining with Ki67 antibodies and SOX2 and TRB2 markers and in 2020 they also tested cyclin D levels. I'm not an expert at this field and I want to know what methods I can use for my experiment. Thank You


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